Evolutionary conservation and emerging functional diversity of the cytosolic Hsp70:J protein chaperone network of Arabidopsis thaliana
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Abstract
- Heat shock proteins of 70 kDa (Hsp70s) partner with structurally diverse Hsp40s (J proteins), generating distinct chaperone networks in various cellular compartments that perform myriad housekeeping and stress-associated functions in all organisms. Plants, being sessile, need to constantly maintain their cellular proteostasis in response to external environmental cues. In these situations, the Hsp70: J protein machines may play an important role in fine-tuning cellular protein quality control. Although ubiquitous, the functional specificity and complexity of the plant Hsp70:J protein network has not been studied. Here, we analyzed the J protein network in the cytosol of Arabidopsis thaliana and, using yeast genetics, show that the functional specificities of most plant J proteins in fundamental chaperone functions are conserved across long evolutionary timescales. Detailed phylogenetic and functional analysis revealed that increased number, regulatory differences, and neofunctionalization in J proteins together contribute to the emerging functional diversity and complexity in the Hsp70:J protein network in higher plants. Based on the data presented, we propose that higher plants have orchestrated their "chaperome," especially their J protein complement, according to their specialized cellular and physiological stipulations.
| Title | Evolutionary conservation and emerging functional diversity of the cytosolic Hsp70:J protein chaperone network of Arabidopsis thaliana |
|---|---|
| Creator | Diwan, D. |
| Gowda, V. | |
| Dobriyal, N. | |
| Raul, S. | |
| Brown, R. E. | |
| Hines, Justin K. | |
| Sahi, C. | |
| Verma, A. K. | |
| Publisher | G3 Genes Genomes Genetics |
| Academic Department | Chemistry |
| Division | Natural Sciences |
| Organization | Lafayette College |
| Date Issued | June 2017 |
| Date Available | 2017-06-29T18:10:27Z |
| Type | Article |
| Language | English |
| Keyword | Hsp40 |
| evolution | |
| J protein | |
| Hsp70 | |
| Arabidopsis thaliana | |
| yeast | |
| Bibliographic Citation | Verma, A. K. (2017 June) "Evolutionary conservation and emerging functional diversity of the cytosolic Hsp70:J protein chaperone network of Arabidopsis thaliana." G3 Genes Genomes Genetics 7 (6): 1941-1954. |
| Standard Identifier | Handle 10385/2265 |
| Permalink | http://hdl.handle.net/10385/2265 |
| Rights Statement |
Creative Commons - Attribution
|
| Rights Holders | Diwan, D. |
| Gowda, V. | |
| Dobriyal, N. | |
| Raul, S. | |
| Brown, R. E. | |
| Verma, A. K. | |
| Sahi, C. | |
| Hines, Justin K. |
Contains
| File | Hines-G3-vol7-2017.pdf | Uploaded 2019-10-20 | Public | Download |
| File | Hines-G3-vol7-2017-supp.pdf | Uploaded 2019-10-20 | Public | Download |
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