Excystation of the encysted metacercariae of Echinostoma trivolvis and Echinostoma caproni in a trypsin-bile salts-cysteine medium and morphometric analysis of the excysted larvae
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Abstract
- A trypsin-bile salts-cysteine (TBC) medium was used to excyst the encysted metacercariae of Echinostoina caproni and Echinostoina trivolvis, 2 allopatric species of Echinostoma. This medium was used to replace a previously used trypsin-bile salts JB) medium that was no longer effective because of the unavailability of the original stocks of trypsin. The TBC medium maintained at 41 C allowed for 68.6% excystation of E. caproni at 1 hr and 57.5% excystation of E. trivolvis at 2 hr. The cysteine reductant in the TBC medium was necessary; if it was omitted, excystation was nil. Morphometric analysis was done on the exeysted metacercariae following fixation of the larvae in hot, 5% neutral buffeted formalin and mounting them on slides in glycerin jelly. Body and organ measurements were made on these larvae. The diameter of the acetabulum of E. caproni was significantly greater than that of E. trivolvis. Likewise, the number and diameter of the excretory concretions found in E. caproni were significantly larger than those of E. trivolvis. Morphometric analysis can be used to distinguish structural differences in closely related allopatric species of Echinostoina.
| Title | Excystation of the encysted metacercariae of Echinostoma trivolvis and Echinostoma caproni in a trypsin-bile salts-cysteine medium and morphometric analysis of the excysted larvae |
|---|---|
| Creator | Fried, Bernard |
| Saxton, T. M. | |
| Peoples, R. C. | |
| Publisher | Journal of Parasitology |
| Academic Department | Biology |
| Division | Natural Sciences |
| Organization | Lafayette College |
| Date Issued | June 2008 |
| Date Available | 2014-04-02T18:17:57Z |
| Type | Article |
| Language | English |
| Bibliographic Citation | Saxton, T. M., B. Fried, and R. C. Peoples (2008 June) "Excystation of the encysted metacercariae of Echinostoma trivolvis and Echinostoma caproni in a trypsin-bile salts-cysteine medium and morphometric analysis of the excysted larvae." Journal of Parasitology 94 (3): 669-671. |
| Standard Identifier | Handle 10385/1424 |
| Permalink | http://hdl.handle.net/10385/1424 |
| Rights Statement |
In Copyright
|
| Rights Holders | American Society of Parasitologists |
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| File | Fried-JournalofParasitology-vol94-no3-2008.pdf | Uploaded 2019-10-20 | Public | Download |
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