The expulsion of Echinostoma trivolvis: Suppressive effects of dexamethasone on goblet cell hyperplasia and worm rejection in C3H/HeN mice

Fried, Bernard; Fukuda, K.; Ichikawa, H.; Fujino, T.

The expulsion of Echinostoma trivolvis: Suppressive effects of dexamethasone on goblet cell hyperplasia and worm rejection in C3H/HeN mice

Abstract

C3H/HeN mice were infected with Echinostoma trivolvis metacercariae on day O, given intramuscular injections of dexamethasone daily for 5 or 7 days, and necropsied on days 5, 8, 12, 15, 20 and 30 p.i. Controls consisted of mice that were infected with echinostomes, but were not treated with dexamethasone. Dexamethasone treatment caused a delay in worm expulsion from the small intestine of the hosts, and the increase in goblet cell numbers that occurred in untreated mice was markedly delayed in the treated mice. Mast cell number in the small intestine increased rapidly from lust after day 5 p.i. and reach ed a peak on day 15 p.i. in both dexamethasone-treated and control mice, although the increase in cell numbers was delayed slightly in the dexamethasone-treated mice. The eosinophil number in the small intestine of dexamethasone-treated mice was suppressed until 8 days p.i. and then increased reaching a peak on day 12 p.i., although the number was about one half that of the control. As determined on day 12 p.i., the mean body area oi worms from dexamethasone-treated animals was significantly greater than that of the controls. Histological examination of the small intestine showed that the goblet and Paneth cell hyperplasia seen in mice infected with E. trivolvis was suppressed by dexamethasone-treatment. Transmission electron microscopy revealed no marked ultrastructural differences in the smell intestine of the dexamethasone-treated and control mice except that the former had an increased number of intracristal granules in mitochondria, an increase in vesicles in the apical epithelial cells and oil increase in amorphous bodies and autophagic vacuoles in the Paneth cells. These results indicate that dexamethasone treatment delayed the expulsion of E. trivolvis from the small intestine of the host mouse in association with the suppression of goblet cell hyperplasia and increase in the number of most cells and eosinophils.

Title	The expulsion of Echinostoma trivolvis: Suppressive effects of dexamethasone on goblet cell hyperplasia and worm rejection in C3H/HeN mice
Creator	Fried, Bernard
	Fukuda, K.
	Ichikawa, H.
	Fujino, T.
Publisher	Parasite
Academic Department	Biology
Division	Natural Sciences
Organization	Lafayette College
Date Issued	1996
Date Available	2018-12-20T19:45:40Z
Type	Article
Language	English
Keyword	goblet cell hyperplasia
	dexamethasone
	worm rejection
	Echinostoma trivolvis
	C3H/HeN mice
Bibliographic Citation	Fujino, T., et al. (1996) "The expulsion of Echinostoma trivolvis: Suppressive effects of dexamethasone on goblet cell hyperplasia and worm rejection in C3H/HeN mice." Parasite 3 (3): 283-289.
Standard Identifier	Handle 10385/2482
Standard Identifier	DOI 10.1051/parasite/1996033283
Permalink	http://hdl.handle.net/10385/2482
Rights Statement	Creative Commons - Attribution
Rights Holders	Fried, Bernard
	Fujino, T.
	Ichikawa, H.
	Fukuda, K.