The expulsion of Echinostoma trivolvis caused by goblet cell hyperplasia in severe combined immunodeficient (SCID) mice

Fried, Bernard; Fujino, T.; Ichikawa, H.; Fukuda, K.

The expulsion of Echinostoma trivolvis caused by goblet cell hyperplasia in severe combined immunodeficient (SCID) mice

Abstract

Mice with severe combined immunodeficiency (SCID), lacking functional T and B lymphocytes, were each infected with 40 Echinostoma trivolvis metacercarial cysts on day 0. The mice of the test group were given intramuscular injections of dexamethasone (DEX) daily for 2 weeks and necropsied on days 5, 8, 12, 15, 20 and 30 post-infection (p. i.). The control mice, not treated with DEX, were each infected with 40 echinostome cysts on day 0 and necropsied on the some days as the DEX-treated mice. In the control mice, worm rejection began about day 8 p. i. and the worms were completely rejected by day 15 p. i., corresponding io the peak in goblet cell hyperplasia, about day 12 p. i. In the DEX-treated mice, goblet cell hyperplasia was significantly suppressed and the worms were retained until day 15 p. i., and then rejected after the last treatment with DEX. The number of mucosal mast cells, that increased with worm infection and peaked about day 15 p, i., was apparently suppressed by treatment with DEX. The eosinophil number in the controls increased on day 15 p. i. approximately and then decreased. The eosinophil number in the DEX-treated mice increased as in the controls, but was significantly suppressed compared to that of the controls during the period of the experiment. Enzyme-linked immunosorbent assay [ELISA] showed no marked rise in titres of the sera IgM, IgA and IgG throughout the experiment in both groups. These results indicate that DEX-treatment delayed the rejection of E. trivolvis from the smell intestine of SCID mice in association with the suppression of goblet cell hyperplasia it is concluded that the host immune system is not involved in the rejection of E. trivolvis and the effector cells far worm rejection are goblet cells that markedly increase in numbers by infection with E. trivolvis.

Title	The expulsion of Echinostoma trivolvis caused by goblet cell hyperplasia in severe combined immunodeficient (SCID) mice
Creator	Fried, Bernard
	Fujino, T.
	Ichikawa, H.
	Fukuda, K.
Publisher	Parasite
Academic Department	Biology
Division	Natural Sciences
Organization	Lafayette College
Date Issued	1998
Date Available	2018-12-20T19:51:48Z
Type	Article
Language	English
Keyword	goblet cell hyperplasia
	worm rejection
	Echinostoma trivolvis
	SCID mice
Bibliographic Citation	Fujino, T., et al. (1998) "The expulsion of Echinostoma trivolvis caused by goblet cell hyperplasia in severe combined immunodeficient (SCID) mice." Parasite 5 (3): 219-222.
Standard Identifier	DOI 10.1051/parasite/1998053219
Standard Identifier	Handle 10385/2483
Permalink	http://hdl.handle.net/10385/2483
Rights Statement	Creative Commons - Attribution
Rights Holders	Fried, Bernard
	Fujino, T.
	Ichikawa, H.
	Fukuda, K.