The effects of different Echinostoma caproni miracidial doses on the lutein and beta-carotene content of Biomphalaria glabrata snails
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Abstract
- The effects of 5, 25, and 40 Echinostoma caproni miracidia
on the lipophilic pigment content of Biomphalaria glabrata snails
was studied using reverse phase high performance thin-layer
chromatography-densitometry. Analysis was done on the whole
body at two and four weeks post exposure. All extraction and
analysis steps were performed in minimal light in order to prevent
pigment degradation. The pigments were extracted with acetone,
evaporated to dryness, reconstituted using heptane, and analyzed
on reverse phase silica gel HPTLC plates with a concentrating zone
using a petroleum ether (37.8-53.5oC)-acetonitrile-methanol (1:1:2)
mobile phase. The analysis yielded significant differences between
the lutein and beta-carotene content of the snails at two and four
weeks post exposure and between the different miracidial doses.
| Title | The effects of different Echinostoma caproni miracidial doses on the lutein and beta-carotene content of Biomphalaria glabrata snails |
|---|---|
| Creator | Fried, Bernard |
| DeGrandchamp, D. | |
| Sherma, Joseph | |
| Publisher | Journal of Veterinary Science and Medical Diagnosis |
| Academic Department | Biology |
| Chemistry | |
| Division | Natural Sciences |
| Organization | Lafayette College |
| Date Issued | October 18, 2015 |
| Date Available | 2016-05-25T18:24:20Z |
| Type | Article |
| Language | English |
| Keyword | Miracidia |
| Echinostoma caproni | |
| Thin-layer chromatography | |
| Pigments | |
| Biomphalaria glabrata | |
| Lutein | |
| Beta-carotene | |
| Bibliographic Citation | DeGrandchamp, D., B. Fried and J. Sherma (2015 Oct 18) "The effects of different Echinostoma caproni miracidial doses on the lutein and beta-carotene content of Biomphalaria glabrata snails." Journal of Veterinary Science and Medical Diagnosis 4 (5): 1-4. |
| Standard Identifier | Handle 10385/2119 |
| DOI 10.4172/2325-9590.1000176 | |
| Permalink | http://hdl.handle.net/10385/2119 |
| Rights Statement |
In Copyright
|
| Rights Holders | SciTechnol |
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| File | Fried-JournalofVeterinaryScienceandMedicalDiagnosis-vol5-2015.pdf | Uploaded 2019-10-20 | Public | Download |
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