Transfer of TLC screening methods to quantitative HPTLC-densitometry methods for pharmaceutical products containing amlodipine besylate, cefpodoxime proxetil, cetirizine 2HCl, diclofenac sodium, efavirenz, mefenamic acid, and atovaquone plus proguanil HCl

Transfer of seven thin-layer chromatography (TLC) Global Pharma Health Fund E.V. Minilab protocols for screening counterfeit pharmaceutical products in the field to quantitative high-performance TLC (HPTLC)-densitometry methods was performed using a model process published previously. The developed and validated methods for tablets containing amlodipine besylate, cefpodoxime proxetil, cetirizine 2HCl, diclofenac sodium, efavirenz, mefenamic acid, and atovaquone + proguanil HCl involved the use of only relatively inexpensive and nontoxic solvents, Merck KGaA Premium Purity HPTLC silica gel 60 F-254 plates, semi-automated sample and standard solution application with a CAMAG Linomat 4, and automated densitometry with a CAMAG Scanner 3 for detection, identification, and quantification. In addition, previously transferred HPTLC-densitometry methods for azithromycin and for cephalexin were used to analyze a new product of each drug to demonstrate the applicability of the methods.

Title	Transfer of TLC screening methods to quantitative HPTLC-densitometry methods for pharmaceutical products containing amlodipine besylate, cefpodoxime proxetil, cetirizine 2HCl, diclofenac sodium, efavirenz, mefenamic acid, and atovaquone plus proguanil HCl
Creator	Sherma, Joseph
	Gu, Y.
	Zeng, B.
Publisher	Acta Chromatographica
Academic Department	Chemistry
Division	Natural Sciences
Organization	Lafayette College
Date Issued	December 2019
Date Available	2019-12-13
Type	Article
Language	English
Keyword	thin-layer chromatography
	TLC
	cefpodoxime proxetil
	counterfeit drug analysis
	cetirizine HCl
	diclofenac sodium
	azithromycin
	amlodipine besylate
	densitometry
	atovaquone plus proguanil HCl
	mefenamic acid
	cephalexin
Bibliographic Citation	Zeng, B. (2019 Dec.) "Transfer of TLC screening methods to quantitative HPTLC-densitometry methods for pharmaceutical products containing amlodipine besylate, cefpodoxime proxetil, cetirizine 2HCl, diclofenac sodium, efavirenz, mefenamic acid, and atovaquone plus proguanil HCl." Acta Chromatographica 31 (4): 246-249.
Standard Identifier	DOI 10.1556/1326.2018.00523
Standard Identifier	Handle 10385/bk128b48h
Permalink	http://hdl.handle.net/10385/bk128b48h
Rights Statement	Creative Commons - Attribution, Non-Commercial
Rights Holders	Sherma, Joseph
	Gu, Y.
	Zeng, B.